Composite
Part:BBa_I715065:Design
Designed by: Malcolm Campbell Group: iGEM07_Davidson_Missouri_W (2009-05-29)
Burnt Pancake Tester with RE and backwards Tet resistance
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1043
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 897
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
Illegal NgoMIV site found at 503
Illegal NgoMIV site found at 871 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2160
Design Notes
With the RE, recombination enhancer, the flipping goes faster. This is not the construct we used in our Burnt Pancake paper.
Source
It comes from subparts in the registry.